Resume                

                       Todd J. Menkhaus

  Condensed Resume

        Condensed Resume (.pdf File)

  Extended Resume

        Extended Resume (.pdf File)

Condensed Resume

TODD J. MENKHAUS, Ph.D.
 

CURRENT POSITION

Chemical and Biological Engineering Department:  South Dakota School of Mines and Technology, Rapid City, SD

Assistant Professor – August 2005 – Present

EDUCATION

Ph.D. Chemical Engineering, December 2002
Iowa State University, Ames, Iowa
Advisor: Dr. Charles E. Glatz

Ph.D. Dissertation: "Recovery and Purification of Recombinant Proteins from Plants: Strategies in Implementation and Optimization"

                        Summary:  Investigated clarification and purification methods to recover recombinant proteins from agricultural plant extracts.  Processes were evaluated for upstream operations (i.e. size reduction, milling, extraction, and centrifugation) and downstream operations (chromatography, precipitation, filtration, and expanded bed adsorption) for a variety of host plants (canola, corn, soybean, and pea).  Results established theoretical and applied guidelines for developing efficient processes for the purification of recombinant industrial, therapeutic, and pharmaceutical products from plant agriculture.

B.S. Chemical Engineering, December 1998
University of Wyoming, Laramie, Wyoming

RELEVANT PUBLICATIONS

1.   Menkhaus, TJ, and Glatz, CE. Antibody Capture from Corn Endosperm Extracts by Packed Bed and Expanded Bed Adsorption. Biotechnology Progress, 21(2), 473 – 485 (2005).

2.   Menkhaus, TJ, Bai, Y, Zhang, CM, Nikolov ZL, and Glatz, CE. Considerations in the recovery of recombinant proteins from transgenic plants (A Review). Biotechnology Progress, 20(4), 1001 – 1014 (2004).  ** The 11^th most-accessed article in Biotechnology Progress during 2004 – 2005.

3.   Menkhaus, TJ, Pate, C, Kretch, A, and Glatz, CE. Recombinant protein purification from pea. Biotechnology and Bioengineering, 86(1), 108 – 114 (2004).

4.   Menkhaus, TJ, and Glatz, CE. Compatibility of Column Inlet and Adsorbent Designs for Processing of Crude Corn Extract by Expanded Bed Adsorption. Biotechnology and Bioengineering, 87(3), 324-336 (2004).

5.   Menkhaus TJ, Eriksson SU, Whitson PB, and Glatz CE. Host selection as a
downstream strategy: Polyelectrolyte precipitation of beta-glucuronidase from plant extracts. Biotechnology and Bioengineering, 77(2), 148-154 (2002).

6.   Menkhaus TJ, Hrma PR, and Li H. Kinetics of nepheline crystallization from high- level waste glass. Ceramic Transactions 107: 461-469 (2000).

PROFESSIONAL EXPERIENCE

Process Development and Pilot Plant Operations: Xoma Pharmaceuticals (US) LLC, Berkeley, CA

Scientist II – April 2004 – August 2005

Summary: Led activities in clarification and purification process development, scale-up, and clinical manufacturing of recombinant proteins produced in microbial and mammalian systems under cGMP compliance.  Processes included: depth filtration, tangential flow filtration (cell separation and UF/DF membrane operations), viral filtration, various modes of chromatography (affinity, ion-exchange, hydrophobic interaction, etc.), and membrane adsorption.

Purification Process Development: Regeneron Pharmaceuticals, Tarrytown, NY

Research Engineer – November 2002 – April 2004
Summary: Developed and implemented methods for the clarification of crude bioreactor cultures and subsequent purification of protein for three antibody fusion products.  Processes were successfully scaled to 12,000 L production.  Also developed and implemented analytical tools (e.g. HPLC, GC, ELISA and colorometric assays), for evaluating protein quality, quantifying product impurities, and supporting bioreactor and media development groups.
 

SELECTED HONORS

-         Awarded the Iowa State University Excellence in Graduate Research honor, Awarded to the top 5% of graduate research at Iowa State University, November 2002

-         Awarded the University of Wyoming Chemical Engineering Department Top Graduate, December 1998

PROFESSIONAL ORGANIZATIONS

American Institute of Chemical Engineers (AIChE)

AIChE, Society for Biological Engineers (SBE)

American Chemical Society (ACS)

COLLABORATORS

Dr. Yun Bai (Applied Molecular Evolution), Ms. Sara Eriksson (Royal Institute of Technology, Stockholm, Sweden), Dr. Charles Glatz (Iowa State Univ.), Mr. Anthony Kretch (Iowa State Univ.), Dr. Zivko Nikolov (Texas A&M Univ.), Ms. Cynthia Pate (Cornell University), Mr. Paul Whitson (Iowa State University), Dr. Chenming Zhang (Virginia Polytechnic Institute)

GRADUATE ADVISOR

Dr. Charles E. Glatz, Professor of Chemical Engineering, Department of Chemical and Biological Engineering, Iowa State University, Ames, IA

  Extended Resume

TODD J. MENKHAUS, Ph.D.
501 E. St. Joseph St.

Rapid City, SD 57701

605-394-2422

Todd.menkhaus@sdsmt.edu

EDUCATION

Ph.D. Chemical Engineering, with Honors, December 2002
Iowa State University, Ames, Iowa
Advisor: Dr. Charles E. Glatz
GPA 3.94/A=4.00

B.S. Chemical Engineering, with Honors, December 1998
University of Wyoming, Laramie, Wyoming
GPA 4.00/ A=4.00

CURRENT POSITION

Chemical and Biological Engineering Department:  South Dakota School of Mines and Technology, Rapid City, SD

Assistant Professor – August 2005 – Present

PROFESSIONAL EXPERIENCE

Process Development and Pilot Plant Operations: Xoma (US) LLC, Berkeley, CA

Scientist II – April 2005 – August 2005

Scientist I – April 2004 – March 2005

Summary: Led activities for selection, process development, scale-up, and clinical manufacturing of harvest and purification operations for recombinant proteins produced in microbial and mammalian systems.  Processes included: depth filtration, tangential flow filtration (cell separation and UF/DF), viral filtration, various modes of chromatography (affinity, ion-exchange, hydrophobic interaction), and membrane adsorbers.

-   Planned, supervised, and performed development activities for recovery and purification

-   Planned, supervised, and performed recovery and purification efforts for clinical production of biologics under cGMP compliance (up to 500 L fermentor scale)

-   Constructed and validated equipment for cGMP use

-   Authored SOPs, Batch Production Records, and technical reports

-   Transfered processes to manufacturing teams

-   Member of project team that developed and implemented a capacity utilization model to effectively utilize resources and pinpoint bottlenecks from gene cloning through clinical manufacturing

-   Communicated with outside collaborators and vendors to move projects forward and implement current practices

-   Discussed project direction and presented experimental results, manufacturing outcomes, and technical seminars to all levels of management and personnel throughout the company and with collaborators

-   Supervised, mentored, motivated and work with associates

November 2002 – April 2004
Research Engineer - Purification Process Development: Regeneron Pharmaceuticals, Inc., Tarrytown, NY
- Developed and implemented methods for the harvest of crude bioreactor cultures and subsequent purification of protein for three antibody fusion products

- Developed and implemented analytical tools (e.g. HPLC, GC, ELISA and colorometric assays), for evaluating protein quality, quantifying product impurities, and supporting bioreactor and media development groups
- Planned, supervised, and performed small-scale experiments to evaluate and optimize process alternatives for clarification and purification of protein products; including centrifugation, depth filtration, chromatography (affinity, ion-exchange, HIC, and size exclusion), and TFF operations (cell separation and UF/DF).

- Processes successfully scaled to 10,000 L (one product) & 1,500 L (two products)
- Performed harvest and purification of protein for small scale pilot manufacturing
(up to 200 L), including clarification, chromatographic and membrane
separations.

- Planned, performed, and interpreted results for scale-down viral clearance evaluation of purification processes
- Transferred process recommendations and provided troubleshooting support for
harvest and purification of products to pilot and manufacturing facilities
- Authored batch records and SOPs for use in process development and
manufacturing  
- Interfaced with all levels of management to discuss project priorities and direction
- Provided work direction, assigned tasks, and guided associates on harvest and purification process development issues, including one direct report.

January 1999 - November 2002
Research Associate: Chemical Engineering Dept., Iowa St. Univ., Ames, IA
Ph.D. Thesis "Recovery and Purification of Recombinant Proteins from Plants: Strategies in Implementation and Optimization"
- Developed and optimized a lab-scale process (with considerations for scale-up) for
the recovery and purification of a human antibody produced in corn. The process
consisted of:
- Processing the plant material, including degermination and particle size
reduction, with pilot scale equipment
- Exploring and implementing conditions for selective extraction of antibody from
corn solids into an aqueous medium
- Evaluating alternative approaches to perform requisite solids clarification
- Assessment of chromatographic resins and binding conditions for use with
expanded bed adsorption (EBA) and packed bed chromatography, including affinity, ion-exchange, hydrophobic interaction, and immobilized metal affinity (IMAC) modes
- model simulations to predict adsorption performance from small-scale lab
experiments
- Comparison of EBA column designs and operating protocols
- Computer process design simulations using (Bio)SuperPro Designer to perform
initial costing estimates and locate potential bottleneck areas
- Investigated the role plant host plays in the efficiency of polyelectrolyte precipitation of "spiked" recombinant protein from plant extracts
- Produced recombinant proteins by shake flask fermentation (up to 4 L), utilizing E. coli as the host organism
- Purified recombinant proteins by preparative scale chromatography  
- Supervised and directed nine undergraduate research assistants
 

 SKILLS and TRAINING
- cGMP Manufacturing experience: centrifugation, depth filtration, TFF, UF/DF, manual and automated chromatography systems, validation of equipment, and general documentation and compliance issues.

- Development/technical skills include an in-depth understanding and experience with: Streamline and UpFront EBA units, Biorad and AKTA Explorer FPLC units, Agilent and Waters HPLC systems, Shimadzu GC, Electrophoresis, UV/Vis spectrophotometers, ELISA, ultracentrifuges, pilot-scale seed processing equipment (i.e. seed milling and flaking), and general laboratory equipment
- Proficient in the use of FORTRAN 90, Microsoft Office, MATLAB, SAS,
and Extend and (Bio)SuperPro Designer bioprocess simulation software
- Successfully completed industrial short courses on "cGMP and Quality Issues for
Biopharmaceuticals", and "Global Leadership Training", and “Time Management for Managers”
- Successfully completed the Fundamentals of Engineering exam

TEACHING and MENTORING EXPERIENCE

SDSM&T Teaching Activities

-         CHE 217: Chemical Engineering I (Material and Energy Balances)

o        Fall 2005: 27 Students

-         CHE/EnvE 318: Chemical Engineering IV (Mass Transfer and Unit Operations)

o        Spring 2006: 15 Students

-         CHE 484/484 and 484L/584L: Biochemical Engineering Lecture and Lab

o        Spring 2006: 14 Students

§         Taught two week bioseparations section

§         Developed and taught two lab units on recovery and separations

-         Graduate Students

o        Master’s

§         Navody Ravikanti: Fall 2005 –

·         Development of Bioseparations Laboratories

-         Graduate Student Committees

o        Spring 2006: One (1)

January 2002 – December 2002

Preparing Future Faculty Fellow, Iowa State Univ., Ames, IA

-   One of 30 graduate students selected campus-wide to participate in a faculty seminar and mentoring series designed to introduce graduate students to life as a professor

-   Attended weekly seminars discussing topics such as balancing faculty expectations, student learning styles, lecturing, technology in the classroom and faculty service

-   Attended weekly mentoring sessions with Dr. Charles Glatz in chemical engineering to discuss topics such as research funding/grant writing, teaching in chemical engineering, and developing class assignments and tests

August 2002 - December 2002
Co-Instructor: Bioseparations, Iowa State Univ. Chem. Eng. Dept., Ames, IA
- Lectured three times weekly for 50 minutes on bioseparations topics
- Developed, assigned, and graded homework, quizzes and tests
- Class of 20 students

 

June 2001 - July 2001
Co-Instructor: ISU Chemical Engineering Department Foreign Study Program,
University College London (UCL), London, England, UK.
- Acted as instructor and chaperone of 30 Iowa State Univ. undergraduate students for unit operations lectures and laboratories at UCL, and industrial tours of the United Kingdom
- Conducted daily tutorial sessions and graded reports, presentations, and projects

January 2001 - July 2001
High School Student Recruiter: Iowa State Univ. Chem. Eng. Dept., Ames, IA
- Member of graduate student team that prepared presentations to explain chemical engineering concepts to high school students
- Traveled to area high schools to give presentations and discuss chemical engineering concepts
- Prepared and gave introductory lectures, and performed lab experiments for the
Engineering Honors Workshop designed to introduce high school students to basic
engineering principles.

January 2000 - May 2000
Teaching Assistant: Chemical Engineering Lab 2, Iowa State University, Ames, IA
- Instructed and managed nine laboratory experiments for undergraduate unit operations class
- Tutored and graded reports for 17 undergraduate chemical engineering students

 

September 1998 - December 1998
Teaching Assistant: Chemistry I Laboratory, Univ. of Wyoming, Laramie, WY
- Directed a weekly laboratory session of 21 undergraduate students
- Responsible for one 50 minute weekly lecture, daily tutorial sessions, helping
students with experiments, and grading laboratory reports, homework
assignments, and exams.
 

PUBLICATIONS

1. Menkhaus, TJ, and Glatz, CE. Antibody Capture from Corn Endosperm Extracts by Packed Bed and Expanded Bed Adsorption. Biotechnology Progress, 21(2), 473 – 485 (2005).

2. Menkhaus, TJ, Bai, Y, Zhang, CM, Nikolov ZL, and Glatz, CE. A review: Considerations in the recovery of recombinant proteins from transgenic plants. Biotechnolgy Progress, 20(4), 1001 – 1014 (2004).  ** The 11^th most-accessed article in Biotechnology Progress during 2004.
3. Menkhaus, TJ, Pate, C, Kretch, A, and Glatz, CE. Recominant protein purification from pea. Biotechnolgy and Bioengineering, 86(1), 108 – 114 (2004).
4. Menkhaus, TJ, and Glatz, CE. Compatibility of Column Inlet and Adsorbent Designs for Processing of Crude Corn Extract by Expanded Bed Adsorption. Biotechnology and Bioengineering, 87(3), 324-336 (2004).
5. Menkhaus TJ, Eriksson SU, Whitson PB, and Glatz CE. Host selection as a
downstream strategy: Polyelectrolyte precipitation of beta-glucuronidase from plant extracts. Biotechnology and Bioengineering, 77(2), 148-154 (2002).
6. Menkhaus TJ, Hrma PR, and Li H. Kinetics of nepheline crystallization from high- level waste glass. Ceramic Transactions 107: 461-469 (2000).
 

PRESENTATIONS

1. Menkhaus, TJ.  Engineering unit operations for recovery of pharmaceutical proteins from transgenic agriculture.  South Dakota School of Mines and Technology Fall 2005 Seminar Series, Rapid City, SD, October, 2005 (invited).

2. Menkhaus TJ and Glatz, CE. Considerations for the application of
expanded bed adsorption in the recovery of a recombinant protein from maize.
Presented at the 224th ACS National Meeting, Boston, MA, August 2002.
3. Menkhaus, TJ. Recovery of recombinant proteins from transgenic plants: Strategies in optimization. Presented at Monsanto, St. Lous, MO, July 2002. (Invited)
4. Menkhaus TJ, Bai Y, and Glatz, CE. Processing strategies for recovery and purification of recombinant proteins from plant hosts. Presented at the AIChE annual meeting, Reno, NV, Novermber 2001.
5. Menkhaus TJ, Zhang C, Bai Y, and Glatz, CE (presenter). Recombinant proteins
from plants - what will this mean for recovery? Presented at IBC International Conference on Recovery and Purification of Biopharmaceuticals, San Diego, CA, November 2001. (Invited).
6. Bai Y, Menkhaus TJ, and Glatz, CE (presenter). Matching unit operations to plant hosts for recovery of recombinat proteins. Poster presented at Recovery of Biological Products X, Cancun, Mexico, June 2001. (Invited)
7. Menkhaus TJ, Eriksson SU, Whitson PB, and Glatz, CE. Polyelectrolyte precipitation of wild-type and charge-modified beta-glucuronidase from corn, canola, and soy extracts. Presented at the AIChE Annual Meeting, Los Angeles, CA, November 2000.
8. Menkhaus TJ, Eriksson SU, Whitson PB, and Glatz, CE. Polyelectrolyte precipitation of wild-type and charge-modified beta-glucuronidase from corn, canola, and soy extracts. Presented at 30th Annual Biochemical Engineering Symposium, Boulder, CO, September 2000. Selected 2nd place best presentation award.

HONORS AND ACTIVITIES